Immunoblotting, Co-IP, and Chromatin Immunoprecipitation

The following antibodies were used for immunoblotting, co-immunoprecipitation and immunostaining: anti-BRCA1 (Ab-1) (Catalog#OP92) mouse mAb (MS110) (Calbiochem, EMD Millipore), anti-pol β, anti-phospho-histone H2A.X (Ser139) clone JBW301 (Millipore, # 05–63), Alexa Fluor 555 Goat anti-mouse antibody (Red) (H+L) (Life Technology, #A-21422), anti-Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (14C10) rabbit mAb (Cell Signaling Technology, # 2118). For chromatin immunoprecipitation (ChIP) assay: H3K9me3 (Millipore, #07–523), H3K4me3 (Millipore, # 07–473) and anti-CENP-A. Pol β antibodies were kindly provided by Dr. Samuel H. Wilson (Laboratory of Structural Biology, NIEHS). CENP-A antibodies were previously described (30 (link)).

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Kononenko A.V., Bansal R., Lee N.C., Grimes B.R., Masumoto H., Earnshaw W.C., Larionov V, & Kouprina N. (2014). A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function. Nucleic Acids Research, 42(21), e164.

Publication 2014

clone JBW301 H3K9me3 H3K4me3

Alexa fluor 555 Anti antibody Antibodies Assay Brca1 Chromatin immunoprecipitation Clone Co immunoprecipitation Glyceraldehyde 3 phosphate dehydrogenase Goat H3k4me3 Histone h2a x Mouse Rabbit

Corresponding Organization :

Other organizations : National Cancer Institute, Indiana University – Purdue University Indianapolis, Indiana University Health, Kazusa DNA Research Institute, Wellcome Centre for Cell Biology, University of Edinburgh

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Variable analysis

independent variables

Anti-BRCA1 (Ab-1) (Catalog#OP92) mouse mAb (MS110)
Anti-pol β
Anti-phospho-histone H2A.X (Ser139) clone JBW301
Alexa Fluor 555 Goat anti-mouse antibody (Red) (H+L)
Anti-Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (14C10) rabbit mAb
H3K9me3
H3K4me3
Anti-CENP-A

dependent variables

Immunoblotting
Co-immunoprecipitation
Immunostaining
Chromatin immunoprecipitation (ChIP) assay

control variables

Pol β antibodies were kindly provided by Dr. Samuel H. Wilson (Laboratory of Structural Biology, NIEHS)
CENP-A antibodies were previously described (30 (link))

Annotations

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