HPLC Quantification of NFD Dissolution
Corresponding Organization : Universidad Complutense de Madrid
Other organizations : University of Portsmouth
Variable analysis
- Dilution ratio of dissolution samples with HPLC mobile phase (1:1 v/v)
- Concentration of NFD eluted from the Thermo BDS Hypersil C18 reverse-phase column
- Chromatographic peak integration for NFD
- HPLC mobile phase composition (methanol: water: acetonitrile, 36:55:9 v/v)
- HPLC equipment (Varian Prostar 230 Solvent Delivery Module, Varian Prostar autosampler 410, Varian Prostar 310 UV-visible detector)
- Chromatography data analysis software (Galaxie Chromatography Data System)
- Reverse-phase column (Thermo BDS Hypersil C18, 200 × 4.6 mm, 5 μm)
- Mobile phase filtration (hydrophilic 0.45 μm filter)
- Mobile phase flow rate (1 mL/min)
- Sample injection volume (50 μL)
- Column temperature (25 °C)
- Detector wavelength (240 nm)
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