To determine the effects of DON on PCa steroidogenesis, cells were treated with 0, 1 or 5 µM DON or DHEA 0–100 nM over 2 or 3 days. Non-treated cells were used as a control. Deoxynivalenol (DON) (Sigma-Aldrich, Saint Louis, MI, USA) and dehydroepiandrosterone (DHEA) (Avanti Polar Lipids, Inc., Alabaster, AL, USA) stock solutions were prepared in ethanol. Stocks were dissolved in experimental medium before use.
The doses and experimental scheme were based on our previous study [8 (link)]. All experiments were carried out with three different pools of cells. Because DHEA can serve as a precursor for the more potent androgens T and DHT [44 (link)], as well as estrogens, its effects on prostate health are of interest.
The doses and experimental scheme were based on our previous study [8 (link)]. All experiments were carried out with three different pools of cells. Because DHEA can serve as a precursor for the more potent androgens T and DHT [44 (link)], as well as estrogens, its effects on prostate health are of interest.
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