Eighty-five 6-week old 100 ± 10 g, male golden hamsters, purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd (SCXK (Beijing) 2016-0011, NO.110011200109811516). The animal care procedures and testing were performed in accordance with the guidelines prepared by the Beijing University of Chinese Medicine Animal Center. Every three hamsters were housed in one cage with a comfortable environment of 25°C ± 1°C, 50%–60% humidity, and 12 h/12 h dark/light cycle. We analyzed the quality control analysis of XZP before the experiments (Supplementary Figure S1 ). Thirteen hamsters were fed with standard diet. At the same time, seventy-two hamsters were fed with high fat diet induced into hyperlipidemia models. Three weeks later, blood was collected from the orbit to test TC and TG. Three hamsters with higher TC and TG in the standard diet group were excluded, and ten hamsters were assigned into the normal control (NC). Fifty hamsters with significantly elevated TC and TG in serum than NC group were randomly split into five groups of ten hamsters each, HFD (treated with water, 1.0 mg/kg/day), Atorvastatin group (Pfizer Inc., NO. DP6613, 2.5 mg/kg/day), low-dose XZP (Guizhou Taihe Pharmaceutical Co., Ltd, NO.20200801-2, XZP-L, 0.225 mg/kg/day), middle-dose XZP (XZP-M, 0.45 mg/kg/day) and high-dose XZP group (XZP-H, 0.9 mg/kg/day). Hamsters of ATVTT and XZP groups were treated at 8:30 every morning for 4 weeks, while hamsters in NC and HFD groups were treated with same water. During the treatment, NC group fed with standard diet, HFD and treatment groups fed with high fat diet. High fat diet was provided by Jiangsu Xietong Pharmaceutical Bio-engineering Co., Ltd (NO. 20201026). It contained 41% standard diet, 20% fructose, 18% lard oil, 15% casein, 2% dicalcium phosphate, 2% mineral mix, 0.5% sodium cholate and 1.5% cholesterol. High fat diet was used for this study containing 41% of total calories (Kcal %) from fat, 20% from proteins, and 39% from carbohydrates.
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