Gordonibacter urolithinfaciens DSM
27213T, Ellagibacter isourolithinifaciens DSM 104140T obtained from the DSMZ culture collection,
and the isolated strain E. bolteae CEBAS
S4A9 were cultivated anaerobically in 5 mL WAM tubes. First, 2 mL
of a diluted aliquot of G. urolithinfaciens DSM 27213T and E. bolteae CEBAS S4A9 strains was transferred to WAM (100 mL). Similarly, 2
mL of diluted aliquots of E. isourolithinifaciens DSM 104140T and E. bolteae CEBAS S4A9 strains was transferred to WAM (100 mL). Finally, EA
dissolved in propylene glycol was added to the 100 mL cultures to
obtain a final concentration of 25 μM. During incubation in
an anoxic environment at 37 °C, aliquots (5 mL) were taken for
HPLC analyses as described below. Incubations were made in triplicate,
and the experiment was repeated twice.
27213T, Ellagibacter isourolithinifaciens DSM 104140T obtained from the DSMZ culture collection,
and the isolated strain E. bolteae CEBAS
S4A9 were cultivated anaerobically in 5 mL WAM tubes. First, 2 mL
of a diluted aliquot of G. urolithinfaciens DSM 27213T and E. bolteae CEBAS S4A9 strains was transferred to WAM (100 mL). Similarly, 2
mL of diluted aliquots of E. isourolithinifaciens DSM 104140T and E. bolteae CEBAS S4A9 strains was transferred to WAM (100 mL). Finally, EA
dissolved in propylene glycol was added to the 100 mL cultures to
obtain a final concentration of 25 μM. During incubation in
an anoxic environment at 37 °C, aliquots (5 mL) were taken for
HPLC analyses as described below. Incubations were made in triplicate,
and the experiment was repeated twice.
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