Immunophenotyping of Lymphocytes and B-cells
Corresponding Organization : Medical University of Graz
Protocol cited in 2 other protocols
Variable analysis
- None explicitly mentioned
- Lymphocyte phenotypes (CD3, CD4, CD8, CD45, CD16, CD56, CD19)
- B-cell phenotypes (CD19, IgD, CD24, CD38, CD27, CD86, CD21, IgM)
- Blood samples were processed within 4 hours for analysis by flow cytometry
- Ethylenediaminetetraacetic acid (EDTA) whole blood was used for lymphocyte phenotyping
- Peripheral blood mononuclear cells (PBMCs) were isolated from lithium heparin whole blood by Ficoll gradient density centrifugation for B-cell phenotyping
- Samples were measured using a FACSLyric flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA)
- Data were analyzed using the FACSSuite (BD Biosciences)
- No positive or negative controls were explicitly mentioned in the protocol
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