The Protease Inhibitor Cocktail Set Ⅲ DMSO Solution (EDTA Free), 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF), aprotinin bovine lung, and leupeptin hemisulfate monohydrate were obtained from Wako. The stocks were dissolved in water. Bestatin and pepstatin A were also obtained from FUJIFILM Wako Pure Chemical Corporation, and the stocks were dissolved in DMSO. E-64 was obtained from Sigma-Aldrich, and the stock was dissolved in water. Each protease was used with the following final concentration : AEBSF 1 mmol / L, aprotinin bovine lung 0.8 µmol / L, E-64 15 µmol / L, leupeptin hemisulfate 20 µmol / L, Bestatin 50 µmol / L, pepstatin A 10 µmol / L.
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Protease Inhibitor Cocktail for Experiments
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Corresponding Organization :
Other organizations : Tokushima University, Fukui Prefectural University, Kitasato University
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Variable analysis
independent variables
- Protease Inhibitor Cocktail Set III DMSO Solution (EDTA Free)
- 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF)
- Aprotinin bovine lung
- Leupeptin hemisulfate monohydrate
- Bestatin
- Pepstatin A
- Not explicitly mentioned
- Final concentration of each protease: AEBSF 1 mmol/L, aprotinin bovine lung 0.8 µmol/L, E-64 15 µmol/L, leupeptin hemisulfate 20 µmol/L, bestatin 50 µmol/L, pepstatin A 10 µmol/L
- Positive controls: Not mentioned
- Negative controls: Not mentioned
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