Culturing Breast Cancer Cell Lines

Cells MCF-7 (ATCC number: HTB-22) and MDA-MB-231(ATCC number: HTB-26) cells were cultured in RPMI-1640 growth medium (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) and Eagle’s Minimum Essential Medium (EMEM) (Euroclone SpA, Via Figino, Italy), respectively. Both RPMI-1640 and EMEM medium were supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) 200 mM L-glutamine, and antibiotics; Penicillin-Streptomycin (100 IU/mL–100 µg/mL). Cells were kept in a humidified 5% CO₂ at 37 °C. Doxorubicin-resistant MCF-7 (MCF-7/DR) and MDA-MB-231 (MDA-MB-231/DR) cell lines used in this work were previously developed in our lab and maintained using similar conditions of wild-type cell lines [46 (link),47 (link)].

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Aljabali A.A., Obeid M.A., Bakshi H.A., Alshaer W., Ennab R.M., Al-Trad B., Al Khateeb W., Al-Batayneh K.M., Al-Kadash A., Alsotari S., Nsairat H, & Tambuwala M.M. (2022). Synthesis, Characterization, and Assessment of Anti-Cancer Potential of ZnO Nanoparticles in an In Vitro Model of Breast Cancer. Molecules, 27(6), 1827.

Publication 2022

RPMI-1640 growth medium

Antibiotics Cell type Doxorubicin Eagle Fetal bovine serum Glutamine Mda mb 231 cell lines Penicillin Streptomycin

Corresponding Organization : University of Ulster

Other organizations : University of Jordan, Al-Ahliyya Amman University

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Variable analysis

independent variables

Cell lines used: MCF-7 (ATCC HTB-22) and MDA-MB-231 (ATCC HTB-26)

dependent variables

Not explicitly mentioned

control variables

Culture conditions: RPMI-1640 and EMEM growth media, supplemented with 10% (v/v) fetal bovine serum, 1% (v/v) 200 mM L-glutamine, and antibiotics (Penicillin-Streptomycin 100 IU/mL–100 µg/mL)
Incubation conditions: Humidified 5% CO2 at 37 °C

controls

Positive control: Wild-type MCF-7 and MDA-MB-231 cell lines
Negative control: Not mentioned

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