We used the Covaris E210 AFA instrument (Woburn, MA) (Quail et al. 2008 (link), 2009 ; Fisher et al. 2011 (link)) to shear the DNA into fragments of a desired length. A previous study on automated 454 library preparation (Lennon et al. 2010 (link)) showed that it is possible to use 96-well PCR plates on the E210, and we adapted the method to another plate type and a shorter fragment size (Supplemental Notes, “DNA Fragmentation”). This reduces consumable costs for the shearing step by ∼90-fold compared with the microTUBE plates provided by Covaris.
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DNA Fragmentation Using Covaris E210
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Other organizations : Harvard University, Broad Institute
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Variable analysis
independent variables
- DNA fragmentation method using the Covaris E210 AFA instrument
- DNA fragment length
- Plate type used for DNA fragmentation
- Fragment size
- Previous study on automated 454 library preparation using the Covaris E210 AFA instrument and 96-well PCR plates
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