Clinical samples — blood, sputum, throat swab, midstream urine, hydrothorax, and ascites — were collected by conventional methods recommended in the clinic. Samples were processed according to the standard operating procedure of our institution. All isolates were identified by the VITEK 2 compact system and VITEK 2 Gram-negative identification card (BioMérieux, Marcy-l’Étoile, France). The microbiology laboratory performed antimicrobial-susceptibility testing of the Gram-negative bacterial isolates based on minimum inhibitory concentration. More detailed methods have been described in our previous studies.13 (link),14 (link)
Protocol detail
Bacterial Identification and Antimicrobial Susceptibility
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Antimicrobial
Ascites
Blood
Gram negative bacterial
Hydrothorax
Minimum inhibitory concentration
Sputum
Susceptibility
Throat
Urine
Corresponding Organization :
Other organizations : Shanghai Jiao Tong University, Shanghai Institute of Hematology, Ruijin Hospital
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Variable analysis
independent variables
- Sample type (blood, sputum, throat swab, midstream urine, hydrothorax, and ascites)
- Bacterial isolate identification
- Antimicrobial susceptibility
- Standard operating procedures for sample processing
- VITEK 2 compact system and VITEK 2 Gram-negative identification card for bacterial identification
- Minimum inhibitory concentration for antimicrobial susceptibility testing
- No specific positive or negative controls were mentioned in the provided information.
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