Baculovirus-Mediated Expression of β2AR

β₂AR was expressed in Sf9 insect cells using the baculovirus method (Expression Systems) and purified as described previously (50 (link)).

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Hilger D., Kumar K.K., Hu H., Pedersen M.F., O’Brien E.S., Giehm L., Jennings C., Eskici G., Inoue A., Lerch M., Mathiesen J.M., Skiniotis G, & Kobilka B.K. (2020). Structural insights into differences in G protein activation by family A and family B GPCRs. Science (New York, N.Y.), 369(6503), eaba3373.

Publication 2020

baculovirus method

Baculovirus Insect Sf9 cells

Corresponding Organization : SLAC National Accelerator Laboratory

Other organizations : Medical College of Wisconsin, Tohoku University

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Variable analysis

independent variables

Expression of β₂AR in Sf9 insect cells using the baculovirus method

dependent variables

Purification of β₂AR

control variables

Purification procedure as described previously (50 (link))

controls

No explicit positive or negative controls are mentioned.

Annotations

This protocol is too short. Unable to provide accurate annotations

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