Microbial Community Analysis of Solar Saltern Sediment

Sediment cores (the top 5 cm), composed mainly of salt crystals and brine, were sampled in June 2016 from the final crystallizer pond Vasca #27 of the solar saltern system Saline della Laguna (Sicily, Italy). Chemical analysis of environmental samples, cultivation conditions, microbial community analysis, genome sequencing and assembly, phylogenomic studies, proteome analysis, analysis of polymer hydrolysis metabolites, quantitative PCR, field emission scanning and transmission electron microscopies and fluorescence microscopy are detailed in SI Appendix, Materials and Methods.

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La Cono V., Messina E., Rohde M., Arcadi E., Ciordia S., Crisafi F., Denaro R., Ferrer M., Giuliano L., Golyshin P.N., Golyshina O.V., Hallsworth J.E., La Spada G., Mena M.C., Merkel A.Y., Shevchenko M.A., Smedile F., Sorokin D.Y., Toshchakov S.V, & Yakimov M.M. (2020). Symbiosis between nanohaloarchaeon and haloarchaeon is based on utilization of different polysaccharides. Proceedings of the National Academy of Sciences of the United States of America, 117(33), 20223-20234.

Publication 2020

Brine Fluorescence microscopy Genome Hydrolysis Microbial community Polymer Proteome Saline Salt Transmission electron microscopies

Corresponding Organization :

Other organizations : National Research Council, Helmholtz Centre for Infection Research, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, The Mediterranean Science Commission, Bangor University, Queen's University Belfast, Russian Academy of Sciences, Immanuel Kant Baltic Federal University, Delft University of Technology, Kurchatov Institute

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Variable analysis

independent variables

Sampling location (final crystallizer pond Vasca #27 of the solar saltern system Saline della Laguna)
Sampling time (June 2016)

dependent variables

Chemical composition of environmental samples
Microbial community composition
Microbial genome sequences and assemblies
Microbial phylogenomic relationships
Microbial proteome
Polymer hydrolysis metabolites
Microbial abundance (quantitative PCR)
Microbial cell morphology (field emission scanning and transmission electron microscopies)
Microbial cell fluorescence (fluorescence microscopy)

control variables

Not explicitly mentioned

controls

Not explicitly mentioned

Annotations

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