Male and female adult C57BL/6J wild-type, VGluT2-ires-Cre29 (link) (Jackson Laboratory, stock #016963) and VGluT2::EYFP (R26 EYFP, Jackson Laboratory #006148) mice were housed with ad libitum access to chow and water on a 12h light cycle and tested during the light phase. All experiments were performed under the UK Animals (Scientific Procedures) Act of 1986 (PPL 70/7652) following local ethical approval.
Protocol detail
Optogenetic Manipulation of Glutamatergic Neurons
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Corresponding Organization :
Other organizations : MRC Laboratory of Molecular Biology
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Variable analysis
independent variables
- Mouse genotype (C57BL/6J wild-type, VGluT2-ires-Cre, VGluT2::EYFP)
- Unspecified
- Sex (male and female adult mice)
- Chow and water access (ad libitum)
- Light cycle (12h light)
- Experimental timing (tested during light phase)
- Positive control: Not specified
- Negative control: Not specified
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