Flavonoid Enrichment from Flower Samples

The dried stamen or whole flower samples (100 mg) were placed in 5 mL quartz tubes equipped with a vapor condenser, and then extracted by means of ultrasound-assisted extraction in 1 mL 90% (v/v) aqEtOH in the USC1200TH ultrasonic bath (Prolabo, Fontenay-sous-Bois, France), following optimized extraction conditions: 30 kHz frequency for 45 min at 45 °C [22 (link)]. The extract was centrifuged for 15 min at 5000× g (Heraeus Biofuge Stratos, Thermo Scientific, Illkirch, France), and then the obtained supernatant was filtered through 0.45 μm nylon syringe membranes (Merck Millipore, Saint-Quentin Fallavier, France). The flavonoid enrichment was obtained through the additional DAX-8 (Merck Millipore, Saint-Quentin Fallavier, France) macroporous resin purification step as described in a previous study [22 (link)].

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Tungmunnithum D., Drouet S, & Hano C. (2022). Validation of a High-Performance Liquid Chromatography with Photodiode Array Detection Method for the Separation and Quantification of Antioxidant and Skin Anti-Aging Flavonoids from Nelumbo nucifera Gaertn. Stamen Extract. Molecules, 27(3), 1102.

Publication 2022

Biofuge Stratos 0.45 μm nylon syringe membranes DAX-8

Bath Flavonoid Membranes Nylon Quartz Resin Syringe Ultrasonic Ultrasound

Corresponding Organization : Physiologie, Ecologie et Environnement

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Variable analysis

independent variables

Extraction method (ultrasound-assisted extraction)
Extraction time (45 min)
Extraction temperature (45 °C)
Extraction solvent (90% (v/v) aqueous ethanol)

dependent variables

Flavonoid enrichment

control variables

Sample mass (100 mg)
Extraction frequency (30 kHz)
Centrifugation time (15 min) and speed (5000× g)
Filtration (0.45 μm nylon syringe membranes)
Additional purification step (DAX-8 macroporous resin)

controls

Positive control: Not mentioned.
Negative control: Not mentioned.

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