A mite antigen, Dermatophagoides farinae extract (Biostir AD), was purchased from Biostir Inc. (Osaka, Japan). Betamethasone and tetracycline were obtained from Tokyo Chemical Industry (Tokyo, Japan). White petrolatum including 5% (w/w) liquid paraffin was employed as the vehicle and used to prepare 0.1% (w/w) betamethasone ointment and 3% (w/w) tetracycline ointment.
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