Bacteria were cultured to OD600 1.0 using TSB medium, and 1 ml of culture was collected by centrifugation and washed twice with PBS. The cultures were incubated in PBS with or without 0.4 mM H2O2 for 30 min. The reaction samples were collected by centrifugation, washed twice with PBS, and broken by Ultrasonic Cell Disruptor (Xinzhi, Ningbo, China). Catalase activity was detected using a catalase assay kit (Beyotime, Shanghai, China).
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