Transcriptome analysis of esophageal cells

GSEA was performed using the fgsea package (Korotkevich and Sukhov) in R version 3.6.3. Unbiased gene ontology analyze was performed with the C5 collection adapted for mouse that contains gene sets annotated by Gene Ontology terms that has been downloaded on http://bioinf.wehi.edu.au/software/MSigDB/. The function “fgseaMultilevel” has been used with ranked fold change values corresponding to Krt8-YFP⁺ cells over Krt8-YFP^- cells from cervical esophagus [abs(LFC) > 2; FDR < 0.05].

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Vercauteren Drubbel A, & Beck B. (2023). Single-cell transcriptomics uncovers the differentiation of a subset of murine esophageal progenitors into taste buds in vivo. Science Advances, 9(10), eadd9135.

Publication 2023

Cells Cervical Esophagus Gene Krt8 Mouse

Corresponding Organization : Institut Belge pour la Sécurité Routière

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Variable analysis

independent variables

Krt8-YFP+ cells over Krt8-YFP- cells from cervical esophagus

dependent variables

Ranked fold change values

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input protocol.

Annotations

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