Isolating Airway Basal Cells for Research

Using Institutional Review Board-approved clinical protocols and after obtaining written informed consent, flexible bronchoscopy was performed to collect large airway epithelial cells by brushing the epithelium of healthy nonsmokers (5 (link)). Pure populations of basal cells (BC) were obtained and characterized as previously described (5 (link)). Both primary and immortalized (BCi-NS1.1 cells) BC were maintained in Bronchial Epithelial Growth Media (BEGM, Lonza, Walkersville, MD) and passaged by seeding at a cell density of 3000 cells/cm² in BEGM (40 (link)).

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Gomi K., Staudt M.R., Salit J., Kaner R.J., Heldrich J., Rogalski A.M., Arbelaez V., Crystal R.G, & Walters M.S. (2016). JAG1-Mediated Notch Signaling Regulates Secretory Cell Differentiation of the Human Airway Epithelium. Stem cell reviews, 12(4), 454-463.

Publication 2016

BEGM

Bronchial Bronchoscopy Cells Clinical protocols Epithelial cells Epithelium Growth media Institutional review board Nonsmokers Populations

Corresponding Organization :

Other organizations : Cornell University

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Variable analysis

independent variables

Flexible bronchoscopy to collect large airway epithelial cells by brushing the epithelium

dependent variables

Characteristics of pure populations of basal cells (BC)

control variables

Healthy nonsmokers

controls

No positive or negative controls were explicitly mentioned.

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