Forty-one bacteriophage genome sequences listed in Table 1 were aligned using the CLC whole genome analysis tool by default parameters (Min. initial seed length = 15; Allow mismatches = yes; Min. alignment block = 100; Min. similarity (0.8); Min. length (0.8)). Average nucleotide identity (ANI) and alignment percentage (AP) were calculated based on the aligned genomes. A heat map was computed based on the previous alignment using the heat-map tool with default parameters (Euclidean distance method and complete cluster linkages). Closely related bacteriophage genomes were selected for further comparative synteny analysis. Dot plots were generated to represent homologous regions, orthologs, genome gaps (GGs), and inversions within the genomes. The evolutionary analyses of the whole genome of the phage fp01 were conducted using MEGAX. The Neighbor-Joining method [35 (link)] with a bootstrap test of 1000 replicates and the Jukes–cantor method [36 ] was utilized to determine the evolutionary distances. The Enterobacteria bacteriophage M13 genome was used as out group for the analysis.
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