Cells were harvested for Western blot analysis as described29 (link).
The anti-ZFP57 (abcam, Cambridge, UK, 1:1000), the anti-MEST (abcam, 1:2000), the anti-β-catenin (abcam, 1:5000), the anti-cyclin D1 (abcam, 1:5000), the anti-c-Myc (abcam, 1:5000), the anti-GAPDH (CellSignaling, Danvers, MA, 1:1000) and the anti-α-Tubulin (abcam, 1:5000) were used as the primary antibodies. GAPDH and α-Tubulin were used as an internal control. A 1:5000–10,000 dilution of the Peroxidase-conjugated Affinipure GOAT anti-Mouse and anti-Rabbit IgG (H + L) (Jackson Immunoresearch, USA) was used as the secondary antibody.
The anti-ZFP57 (abcam, Cambridge, UK, 1:1000), the anti-MEST (abcam, 1:2000), the anti-β-catenin (abcam, 1:5000), the anti-cyclin D1 (abcam, 1:5000), the anti-c-Myc (abcam, 1:5000), the anti-GAPDH (CellSignaling, Danvers, MA, 1:1000) and the anti-α-Tubulin (abcam, 1:5000) were used as the primary antibodies. GAPDH and α-Tubulin were used as an internal control. A 1:5000–10,000 dilution of the Peroxidase-conjugated Affinipure GOAT anti-Mouse and anti-Rabbit IgG (H + L) (Jackson Immunoresearch, USA) was used as the secondary antibody.
Free full text: Click here
