Protocol detail

Quantifying Schmallenberg Virus in Culicoides

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Total nucleic acid was extracted from the homogenates of Culicoides heads using a Universal Biorobot (Qiagen) with a QIAamp® All Nucleic Acid MDx Kit (Qiagen) following the manufacturer’s recommended instructions. Schmallenberg virus RNA in the resultant extractions was the assessed using a semi-quantitative RT-qPCR targeting the S segment of the genome [1 , 20 (link)]. A C_q cut-off value of < 35 was used to define SBV infection [15 (link)].

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Barber J., Harrup L.E., Silk R., Veronesi E., Gubbins S., Bachanek-Bankowska K, & Carpenter S. (2018). Blood-feeding, susceptibility to infection with Schmallenberg virus and phylogenetics of Culicoides (Diptera: Ceratopogonidae) from the United Kingdom. Parasites & Vectors, 11, 116.

Publication 2018

Universal Biorobot QIAamp® All Nucleic Acid MDx Kit

Culicoides Genome Heads Infection Nucleic acid Virus rna

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Presence/absence of Schmallenberg virus RNA in the resultant extractions, as assessed using a semi-quantitative RT-qPCR targeting the S segment of the genome

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified
A C[sub]q[/sub] cut-off value of < 35 was used to define SBV infection

Annotations

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