Antibiotic-resistant Bacterial Inhibition

Methanol (CH₃OH, 99.9%), silver nitrate (AgNO₃), nutrient agar, ferric chloride hexahydrate (FeCl₃, 6H₂O), iron (II) sulfate tetrahydrate (FeSO₄.4H₂O), dichloromethane, polyethylene glycol 300, ascorbic acid and Mueller-Hinton agar (MHA) were purchased from Merck (Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and 2,4,6-Tripyridyl-s-Triazine (TPTZ) were purchased from Sigma (Germany) and poly lactic acid (PLA) from Zhejiang Hisun Biomaterials (China). The RAW264 macrophage cell line was purchased from National Cell Bank, Pasteur Institute of Iran, Iran and Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal Bovine Serum (FBS), 1% Penicillin-Streptomycin from Gibco (Paisley, UK). All aqueous solutions were prepared using double distilled water. All reagents used were of analytical grade. Clinical strains of Pseudomonas aeruginosa^{38 (link)} and Staphylococcus aureus^{39 (link)} bacteria were isolated from hospitalized patients by our group, which have been characterized as antibiotic-resistant strains.

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Bardania H., Mahmoudi R., Bagheri H., Salehpour Z., Fouani M.H., Darabian B., Khoramrooz S.S., Mousavizadeh A., Kowsari M., Moosavifard S.E., Christiansen G., Javeshghani D., Alipour M, & Akrami M. (2020). Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing. Scientific Reports, 10, 6129.

Publication 2020

Methanol nutrient agar ferric chloride hexahydrate iron (II) sulfate tetrahydrate dichloromethane polyethylene glycol 300 ascorbic acid Mueller-Hinton agar 2,2-diphenyl-1-picrylhydrazyl 2,4,6-Tripyridyl-s-Triazine Penicillin-Streptomycin

Agar Antibiotic Ascorbic acid Bacteria Biomaterials Bromide Cell Cell line Dichloromethane Diphenyl Eagle Ferric chloride hexahydrate Fetal bovine serum Iron Macrophage Methanol Nutrient Patients Penicillin Poly lactic acid Polyethylene glycol 300 Pseudomonas Silver nitrate Staphylococcus Strains Streptomycin Sulfate Triazine

Corresponding Organization :

Other organizations : Yasuj University of Medical Sciences, Tarbiat Modares University, Jahrom University of Medical Sciences, Aarhus University, Tehran University of Medical Sciences

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Variable analysis

independent variables

Methanol (CH3OH, 99.9%)
Silver nitrate (AgNO3)
Ferric chloride hexahydrate (FeCl3, 6H2O)
Iron (II) sulfate tetrahydrate (FeSO4.4H2O)
Dichloromethane
Polyethylene glycol 300
Ascorbic acid
2,2-diphenyl-1-picrylhydrazyl (DPPH)
3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)
2,4,6-Tripyridyl-s-Triazine (TPTZ)
Poly lactic acid (PLA)

dependent variables

Antioxidant activity
Cytotoxicity
Antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus

control variables

Nutrient agar
Mueller-Hinton agar (MHA)
Dulbecco's Modified Eagle's Medium (DMEM)
Fetal Bovine Serum (FBS)
1% Penicillin-Streptomycin
RAW264 macrophage cell line

positive controls

No information provided

negative controls

No information provided

Annotations

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