Viscosity Analysis of Digesta from Distal Jejunum

Digesta collected from the distal jejunum were divided into two tubes (15 mL) and centrifuged at 1,000 × g at 4 °C for 10 min. The supernatant of each tube was collected into 2 mL tubes and centrifuged at 10,000 × g at 4 °C for 10 min. The supernatant was then transferred to a new 2-mL tube and kept on ice for the measurement of viscosity. A viscometer (Brookfield Digital Viscometer, Model DV-II Version 2.0, Brookfield Engineering Laboratories Inc., Stoughton, MA) was then used to measure the digesta viscosity at 25 °C. The viscosity was calculated as the average between 45.0/s and 22.5/s shear rates and recorded as millipascal-seconds (mPa.s) (Duarte et al., 2019 (link); Chen et al., 2020 (link)).