CXCL12 and HMGB1 Protein Protocol

CXCL12 was chemically synthesized as previously described (35 (link)). Full length HMGB1 and HMGB1-Histidine-tagged were produced and stored in phosphate-buffered saline (PBS; D8537, Sigma Aldrich, Saint Louis, MO, United States) at the Institute of Research in Biomedicine Protein Facility (Bellinzona, Switzerland), or purchased (1690-HMB, R&D Systems, Minneapolis, MN, United States). The chosen sub-optimal concentrations of CXCL12 and the concentration of HMGB1 in each experiment were based on our previous findings (6 (link)).

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D’Agostino G., Artinger M., Locati M., Perez L., Legler D.F., Bianchi M.E., Rüegg C., Thelen M., Marchese A., Rocchi M.B., Cecchinato V, & Uguccioni M. (2020). β-Arrestin1 and β-Arrestin2 Are Required to Support the Activity of the CXCL12/HMGB1 Heterocomplex on CXCR4. Frontiers in Immunology, 11, 550824.

Publication 2020

D8537 1690-HMB

Cxcl12 Histidine Hmgb1 Phosphate Protein Saline

Corresponding Organization : Humanitas University

Other organizations : Biotechnology Institute Thurgau, University of Milan, Istituti di Ricovero e Cura a Carattere Scientifico, University of Bern, Vita-Salute San Raffaele University, University of Fribourg, Medical College of Wisconsin, University of Urbino

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Variable analysis

independent variables

Concentration of CXCL12
Concentration of HMGB1

dependent variables

Not explicitly mentioned

control variables

Phosphate-buffered saline (PBS; D8537, Sigma Aldrich, Saint Louis, MO, United States)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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