FPTLow, which was described in the previous report34 (link), and TR-Dex (D1828, Thermo Fisher), which was used as a cell tracer, were dissolved in an aqueous solution (0.5% w/v) containing 130 mM KCl, 10 mM K2HPO4, and 22 mM NaCl. The solution was filtered using an Ultrafree-MC filter (Millipore, Burlington, MA) and microinjected into the cytoplasm using a glass capillary needle (Femtotips II, Eppendorf, Hamburg, Germany). The volume of the injected solution was estimated to be 2 fl.
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