Myceliophthora thermophila was cultured in 1× VMM supplemented with 2% Avicel, 2% xylan, or AX to induce the production of cellulases or hemicellulases. The protein concentration in the supernatant was determined using the Bio-Rad Protein Assay kit (absorbance of 595 nm) (Bio-Rad, Hercules, CA, USA). The hemicellulose/cellulose hydrolysis reaction system included the condensed culture (50 μg protein), 2% substrate (Avicel or xylan), and 1 μg/mL sodium azide in 1 mL 1× VMM. The samples were incubated at 45 °C for 24 h with shaking at 150 rpm and then centrifuged (14,000 rpm for 15 min at 4 °C). The supernatants were filtered through a 0.22 μm membrane and analyzed using an HPLC system.