Protocol detail

CDK5 Gene Expression Profiling

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CDK5 gene expression was studied by qPCR in cohort C as previously described [14 (link),28 (link)]. Briefly, retrotranscription was performed with moloney murine leukemia virus (MMLV) reverse transcriptase (Thermo Fisher Scientific, Waltham, MA, USA) and the Cdk5 assay no. Hs00762869_s1 was used (Thermo Fisher Scientific, Waltham, MA, USA). Relative gene expression quantification was calculated according to the comparative Ct method as described elsewhere using β-Actin (Thermo Fisher Scientific, Waltham, MA, USA) as the endogenous control.

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Ruiz de Porras V., Bystrup S., Cabrero-de las Heras S., Musulén E., Palomero L., Alonso M.H., Nieto R., Arango D., Moreno V., Queralt C., Manzano J.L., Layos L., Bugés C, & Martinez-Balibrea E. (2019). Tumor Expression of Cyclin-Dependent Kinase 5 (Cdk5) Is a Prognostic Biomarker and Predicts Outcome of Oxaliplatin-Treated Metastatic Colorectal Cancer Patients. Cancers, 11(10), 1540.

Publication 2019

moloney murine leukemia virus (MMLV) reverse transcriptase β-Actin

Actin Assay Cdk5 Gene expression Moloney murine leukemia virus Reverse transcriptase

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Variable analysis

independent variables

CDK5 gene expression

dependent variables

Relative gene expression quantification

control variables

β-Actin (Thermo Fisher Scientific, Waltham, MA, USA) as the endogenous control

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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