Conditional Knockout Mice for Circadian Research

Bmal1^fl/fl, CAG-Cre^ERT2, TH-Cre, Aldh1l1-Cre^ERT2, Cx3cr1-Cre^ERT2, and Nestin-Cre mice were all originally obtained from The Jackson Laboratory and were crossed in our colony. CaMK2a-iCre;Bmal1^fl/fl were previously described (22 (link), 34 (link)), and fixed/frozen tissue were prepared in house. All mice were maintained on a C57BL/6J background. All Cre^ERT2 lines were treated with tamoxifen (MilliporeSigma) at 2 months of age. Mice were given daily i.p. injections of tamoxifen in corn oil at a dose of 2 mg/day for 5 consecutive days. Both Cre⁺ and Cre^– mice were treated with tamoxifen. In all cases, Cre⁺ mice were hemizygous for Cre. Male and female mice were used in all experiments, as we have not observed sex differences in any of the measured endpoints.

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Kanan M.K., Sheehan P.W., Haines J.N., Gomez P.G., Dhuler A., Nadarajah C.J., Wargel Z.M., Freeberg B.M., Nelvagal H.R., Izumo M., Takahashi J.S., Cooper J.D., Davis A.A, & Musiek E.S. (2024). Neuronal deletion of the circadian clock gene Bmal1 induces cell-autonomous dopaminergic neurodegeneration. JCI Insight, 9(2), e162771.

Publication 2024

CAG-CreERT2 TH-Cre Aldh1l1-CreERT2 Cx3cr1-CreERT2 Nestin-Cre tamoxifen

Corresponding Organization :

Other organizations : Washington University in St. Louis, The University of Texas Southwestern Medical Center

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Variable analysis

independent variables

Cre lines (CaMK2a-iCre, CAG-Cre, TH-Cre, Aldh1l1-Cre, Cx3cr1-Cre, Nestin-Cre)
Bmal1 genotype (Bmal1^(fl/fl))

dependent variables

Not explicitly mentioned

control variables

C57BL/6J background
Tamoxifen treatment (both Cre+ and Cre- mice)
Sex (male and female mice used)

controls

Positive control: CaMK2a-iCre;Bmal1^(fl/fl) mice (previously described in references 22 and 34)
Negative control: Cre- mice treated with tamoxifen

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