CTL nanogel was prepared according to the protocol given by Ameena et al.32 (link). The lauric acid solution was prepared by dissolving 0.5 g of lauric acid in 10 mL of ethyl alcohol, While the thiocolchicoside solution was prepared by dissolving 50 mg of thiocolchicoside in 10 mL of distilled water. Three more different formulations of Lauric acid and Thiocochicoside were prepared: 1gm Lauric acid and 50 mg Thiocolchicoside, 0.5gm Lauric acid and 100 mg Thiocolchicoside, and 0.25 g Lauric acid and 150 mg Thiocolchicoside respectively.
The solutions were uniformly mixed using a vortex mixer for 15 min and kept in an orbital shaker overnight at 110 rpm. Chitosan was prepared by adding 0.5 g of medium molecular weight chitosan to 49.5 mL of distilled water. 0.5 mL of glacial acetic acid was added, and the resultant chitosan mixture was stirred continuously for about 2–3 h using a magnetic stirrer at 800 rpm to form a clear solution. The Thiocolchicoside-Lauric acid nanogel was prepared by adding 5 mL of prepared lauric acid solution to 5 mL of prepared thiocolchicoside solution. The reaction mixture was kept on a magnetic stirrer at 700 rpm for 2 h. 10 mL of medium molecular-weight chitosan was added, and the chitosan-mediated nanogel was stirred for up to 24 h to attain uniform dispersion.
The solutions were uniformly mixed using a vortex mixer for 15 min and kept in an orbital shaker overnight at 110 rpm. Chitosan was prepared by adding 0.5 g of medium molecular weight chitosan to 49.5 mL of distilled water. 0.5 mL of glacial acetic acid was added, and the resultant chitosan mixture was stirred continuously for about 2–3 h using a magnetic stirrer at 800 rpm to form a clear solution. The Thiocolchicoside-Lauric acid nanogel was prepared by adding 5 mL of prepared lauric acid solution to 5 mL of prepared thiocolchicoside solution. The reaction mixture was kept on a magnetic stirrer at 700 rpm for 2 h. 10 mL of medium molecular-weight chitosan was added, and the chitosan-mediated nanogel was stirred for up to 24 h to attain uniform dispersion.
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