Immunoblotting was performed as previously described17 (link). Antibodies to the following proteins were used in the immunoprecipitation and immunoblots: SMARCC1/BAF155 (1:3000 dilution, Santa Cruz: sc9746); ARID1A (1:3000 dilution, Cell Signaling Technology: 12354 and home-made); ARID1B (1:1000 dilution, Abcam: ab54761); SMARCA4/BRG1 (1:500 dilution, Santa Cruz: sc17796 and home-made); SMARCC2/BAF170 (1:3000 dilution, Bethyl Laboratories: A301-039A); SMARCD1/BAF60A (1:3000 dilution, Bethyl Laboratories: A301-595A); SMARCE1/ BAF57 (1:3000 dilution, Bethyl Laboratories: A300-810A); SMARCB1/SNF5 (1:3000 dilution, Bethyl Laboratories: A301-087A); ACTL6A/BAF53A (1:3000 dilution, Bethyl Laboratories: A301-391A); ACTIN-HRP (1:3000 dilution, Santa Cruz: sc-47778).
Protocol detail
Immunoblotting of Chromatin Remodeling Proteins
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ARID1A
ARID1B
SMARCA4/BRG1
SMARCC2/BAF170
SMARCD1/BAF60A
SMARCE1/ BAF57
SMARCB1/SNF5
ACTL6A/BAF53A
ACTIN-HRP
A301
Actin
Antibodies
Arid1a
Baf170
Baf57
Baf60a
Brg1
Dilution
Immunoblots
Immunoprecipitation
Proteins
Smarcc1
Snf5
Corresponding Organization :
Other organizations : Dartmouth College, Baylor College of Medicine
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Variable analysis
independent variables
- Antibodies to the following proteins were used in the immunoprecipitation and immunoblots: SMARCC1/BAF155, ARID1A, ARID1B, SMARCA4/BRG1, SMARCC2/BAF170, SMARCD1/BAF60A, SMARCE1/ BAF57, SMARCB1/SNF5, ACTL6A/BAF53A, ACTIN-HRP
- Not explicitly mentioned
- Not explicitly mentioned
- No positive or negative controls were explicitly mentioned.
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