mPDAC cells 9091 and 8661 were plated in 10 cm dishes. The next day, they were treated for 6 hours with DMSO (vehicle) or T/N (10 nM trametinib and 2 μM nintedanib) and analyzed using the Phospho Explorer antibody microarray, which contains 1,318 antibodies (Full Moon Biosystems), according to the protocol of the manufacturer.
Pathway enrichment analysis was based on the Reactome gene-set and performed through Cytoscape (v3.8.2) with ClueGO (v2.5.8)66 (link)
, a Cytoscape plug-in to decipher functionally grouped pathway annotation networks. The functionally grouped networks used for visualization present terms as nodes and are linked based on their kappa score level (≥0.4). The node size represents the term-enrichment significance and functionally related groups are depicted by similar colors.
Pathway enrichment analysis was based on the Reactome gene-set and performed through Cytoscape (v3.8.2) with ClueGO (v2.5.8)66 (link)
, a Cytoscape plug-in to decipher functionally grouped pathway annotation networks. The functionally grouped networks used for visualization present terms as nodes and are linked based on their kappa score level (≥0.4). The node size represents the term-enrichment significance and functionally related groups are depicted by similar colors.
