Tissues were fixed in 4% paraformaldehyde, dehydrated into methanol, infiltrated with paraffin, and cut into 5 μm sections. Sections were deparaffinized in xylene, hydrated, and boiled in 10 mM sodium citrate (pH 6.0) for 20 min. Tissues were washed with a solution containing 25 mM Tris-HCl, pH 7.5, 140 mM NaCl, 2.7 mM KCl, and 0.1% Tween-20 (TBSTw) and non-specific binding sites were blocked for 1 hr in TBSTw containing 1% Blocking Reagent (Roche Diagnostics), 5% normal goat sera, and 1% bovine serum albumin fraction 5 (RGBTw). Tissues were incubated overnight at 4°C with primary antibodies diluted in RGBTw as follows: 1:250 rabbit anti-AR (Santa Cruz Biotechnology, Santa Cruz, CA), 1:250 rabbit anti-CDH1 (Cell Signaling Technologies, Beverly MA), 1:50 mouse anti-KRT14 (Thermo Fisher Scientific, Waltham MA), 1:250 mouse anti-TRP63 (Santa Cruz Biotechnology). After several washes with TBSTw, tissues were incubated for 1 hr with RGBT containing 1:250 diluted fluorescent secondary antibodies (Dylight 488- and 405- conjugated goat anti-mouse IgG, Dylight 546-counjugated goat anti-rabbit IgG, Jackson Immunoresearch (West Grove, PA). For sections that were stained with two primary antibodies from the same host species, unlabeled secondary antibodies (goat anti-mouse or goat anti-rabbit IgG, Jackson Immunoresearch) were used to block antigenic sites prior to introducing the second primary antibody. Labeled tissue sections were counterstained with 4′,6-diamidino-2-phenylindole dilactate and mounted in anti-fade media (phosphate-buffered saline containing 80% glycerol and 0.2% n-propyl gallate). Immunofluorescent staining of ISH-stained sections. ISH stained tissue sections were fixed overnight in 4% PFA and bleached with TBSTw containing 6% hydrogen peroxide. Tissues were blocked for 1 h in RGBTw and then incubated overnight at 4°C in RGBTw containing rabbit anti-CDH1 (1:500, Cell Signaling Technologies) and mouse anti-ACTA2 (1:300, Leica Microsystems, Bannockburn, IL). Tissues were stained with secondary antibodies (1:500 Dylight 488-goat anti-mouse IgG, 1:500 Dylight 546- counjugated goat anti-rabbit IgG, Jackson Immunoresearch) and mounted in anti-fade media. Brightfield and fluorescent images were captured using an Eclipse E600 compound microscope (Nikon Instruments Inc., Melville, NY) and merged using NIS elements imaging software (Nikon Instruments Inc.)