3D Video-oculography for Vestibulo-ocular Reflex

To assess the efficacy of electrical stimulation we used real-time, 3-dimensional video-oculography (3D VOG) to record eye movements associated with the vestibulo-ocular reflex (VOR). This system has been previously described in detail [1 , 4 (link), 5 (link)]. The left eye was topically anesthetized via application of proparacaine (5 mg/mL; Bausch & Lomb, Rochester, NY) and the pupil constricted via pilocarpine (10 mg/mL; Bausch & Lomb, Rochester, NY) eye drops. A marker consisting of three fluorescent yellow squares on a black film was placed on the cornea using veterinary tissue glue (VetOne, Boise, ID) and illuminated via four UV-emitting diodes. A USB3 Camera (FL3-U3– 13Y3M-C, Flea3 1.3 MP Mono USB3 Vision; Point Grey, BC, Canada) retrofitted with a 1/4” format, 16.0mm-focal length, f/2.0 C-mount board lens (BL160; Allthings Inc., Australia) was used to acquire 496 400 pixel, 8-bit gray-scale images at 200 Hz using custom software written in LabVIEW (National Instruments, Austin, TX). This system captures 2-dimensional movement of the illuminated eye marker and outputs 3-dimensional eye velocities corresponding to the axes of the three semicircular canals: horizontal (H), left anterior/right posterior (LARP), and right anterior/left posterior (RALP) [6 (link)].

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Aplin F.P., Singh D., Santina C.C, & Fridman G.Y. (2019). Combined ionic direct current and pulse frequency modulation improves the dynamic range of vestibular canal stimulation. Journal of vestibular research : equilibrium & orientation, 29(2-3), 89-96.

Publication 2019

proparacaine pilocarpine LabVIEW

Austin Axes Cornea Electrical stimulation Eye drops Eye movement Lens Pilocarpine Proparacaine Pupil Semicircular canals Tissue Vestibulo ocular reflex Vision

Corresponding Organization : Johns Hopkins Medicine

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Variable analysis

independent variables

Electrical stimulation

dependent variables

Eye movements associated with the vestibulo-ocular reflex (VOR)

control variables

Topical anesthesia of the left eye via application of proparacaine
Pupil constriction via pilocarpine eye drops
Placement of a marker consisting of three fluorescent yellow squares on the cornea using veterinary tissue glue
Illumination of the eye marker via four UV-emitting diodes
Video-oculography system using a USB3 camera and custom software written in LabVIEW

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