Genetic Analysis of Streptomyces sp. RM-5-8

The genetic DNA of Streptomyces sp. RM-5–8 was previously isolated and sequenced.^{20 (link)} All primers were purchased from Integrated DNA Technologies, and E. coli 5α and BL21(DE3) competent cells were purchased from New England Biolabs. All DNA sequencing was conducted with the primers T7 promoter and T7 terminator (Table S3). Crystallization screens Index HT, PEGRx HT, Crystal Screen HT, and SaltRx HT were obtained from Hampton Research. Rigaku Wizard Classic screens were purchased from Molecular Dimensions. N-(2-hydroxyethyl)-piperazine-N′-ethanesulfonic acid (HEPES) buffer was purchased from Gold Biotechnology in a sodium salt form. All other reagents and chemicals were purchased from Sigma-Aldrich or Fisher Scientific and were used without further purification unless otherwise stated. PD-10 columns and Ni-NTA superflow columns were purchased from GE Healthcare. All solvents used were of ACS grade and purchased from Fisher Scientific or Pharmco-AAPER.