Evaluating Immune Profiles in Rectal Cancer

Sections of archival formalin-fixed paraffin-embedded (FFPE) tissues from 50 patients with rectal cancer (untreated endoscopy biopsy samples at diagnosis and paired posttreatment surgical samples) were analyzed by IHC for CD73, CD8, and Pan-cytokeratin using the Ventana Benchmark Ultra platform (PR_PATMO_0010) as described previously and in the Supplementary Data (25 (link)). A total of 7 samples did not show residual tumor post-chemoradiation and were excluded from the analysis.

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Wurm M., Schaaf O., Reutner K., Ganesan R., Mostböck S., Pelster C., Böttcher J., de Andrade Pereira B., Taubert C., Alt I., Serna G., Auguste A., Stadermann K.B., Delic D., Han F., Capdevila J., Nuciforo P.G., Kroe-Barrett R., Adam P.J., Vogt A.B, & Hofmann I. (2021). A Novel Antagonistic CD73 Antibody for Inhibition of the Immunosuppressive Adenosine Pathway. Molecular Cancer Therapeutics, 20(11), 2250-2261.

Publication 2021

Benchmark Ultra platform

Biopsy Cd73 Chemoradiation Cytokeratin Diagnosis Embedded paraffin Endoscopy Formalin Patients Rectal cancer Residual tumor Surgical

Corresponding Organization :

Other organizations : Boehringer Ingelheim (Austria), Boehringer Ingelheim (Spain), Boehringer Ingelheim (Germany), Universitat Autònoma de Barcelona, Centro de Investigación Biomédica en Red de Cáncer

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Variable analysis

independent variables

Formalin-fixed paraffin-embedded (FFPE) tissue sections
Untreated endoscopy biopsy samples at diagnosis
Paired posttreatment surgical samples

dependent variables

Expression of CD73
Expression of CD8
Expression of Pan-cytokeratin

control variables

Ventana Benchmark Ultra platform (PR_PATMO_0010)
Immunohistochemistry (IHC) analysis

controls

7 samples that did not show residual tumor post-chemoradiation were excluded from the analysis

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