Docosahexaenoic acid (DHA) was obtained from Cayman Chemical (Ann Harbor, MI), RPMI and DMEM media and fetal bovine serum (FBS) from Invitrogen (Carlsbad, CA), and mouse interleukin 4 (IL-4) from Sigma Chemical (St Louis, MO, USA). Coelenterazine was purchased from MedChem Express (Monmouth Junction, NJ). Mouse diets were purchased from DYETS, Inc. (Bethlehem, PA). For the ω-6 diet, 30% of energy (134 g/kg) was provided by corn oil, and the ω-6 to ω-3 ratio was 18:1. For the ω-3 diet 30% of energy was provided by menhaden oil (134 g/kg) and the ω-6 to ω-3 ratio was 1:8 as previously described [5 (link)].
Protocol detail
Dietary Omega-3 and Omega-6 Fatty Acids
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Cayman
Coelenterazine
Corn oil
Diet
Docosahexaenoic acid
Fetal bovine serum
Interleukin 4
Menhaden oil
Mouse
Corresponding Organization : VA Greater Los Angeles Healthcare System
Other organizations : University of Southern California
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Variable analysis
independent variables
- Dietary omega-6 fatty acid (30% of energy from corn oil, omega-6 to omega-3 ratio of 18:1)
- Dietary omega-3 fatty acid (30% of energy from menhaden oil, omega-6 to omega-3 ratio of 1:8)
- Not explicitly mentioned
- Docosahexaenoic acid (DHA) source
- Cell culture media (RPMI and DMEM)
- Fetal bovine serum (FBS)
- Mouse interleukin 4 (IL-4)
- Coelenterazine
- Positive control: Not specified
- Negative control: Not specified
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