The cells were first transfected with lentiviral GFP and then selected by FACS to obtain a high yield of GFP+ cells. A total of 5 × 104 GFP+ DFSCs and Wnt5a+/GFP+ DFSCs in 100 μl DMEM were loaded into 8 μm pore Transwells (Corning, Corning, NY, USA) in 24-well plates as per our prior methods [15 (link)]. Following 12-hour incubation, migrated cells were trypsinized and counted, as per our prior methods [11 (link)].
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