Ex-Taq DNA polymerase, a genomic DNA extraction kit, pGEM-T easy vector, and reagents for polymerase chain reaction were purchased from Promega (Madison, WI, USA). T4 DNA ligase and restriction enzymes were purchased from New England Biolabs (Ipswich, MA, USA). The pET28a expression vector, plasmid isolation kit, and nickel-nitrilotriacetic acid superflow column for His-tag protein purification were obtained from Qiagen (Hilden, Germany)20 (link). Oligonucleotide primers were obtained from Bioneer (Daejeon, Republic of Korea)29 (link). Electrophoresis reagents were provided by Bio-Rad (Hercules, CA, USA), and all chemicals used in assays were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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