To measure leukocyte-mediated cytotoxicity in rat MSCs, the leukocytes were isolated from spleen (SD rat) using HISTOPAQUE 1083 (Sigma-Aldrich) and co-cultured with allogeneic MSCs in the ratio of 10:1 as described in our previous studies12 (link),51 (link). After 72 h of co- culture, leukocyte-mediated cytotoxicity in the MSCs was assessed by measuring the LDH released from the damaged MSCs (LDH Cytotoxicity Detection Kit; Clontech).
To measure leukocyte-mediated cytotoxicity in human MSCs, hMSCs were co-cultured with leukocytes isolated from peripheral blood derived from healthy individuals at a ratio 1:10 for 72 h.
To measure leukocyte-mediated cytotoxicity in human MSCs, hMSCs were co-cultured with leukocytes isolated from peripheral blood derived from healthy individuals at a ratio 1:10 for 72 h.
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