The concentration of RNA was measured with a Nanodrop 2000 UV-VIS spectrophotometer (Thermo Scientific, Wilmington, DE) and expressed as nanograms per microliter. The integrity of the RNA samples was assessed by sample preparation using Agilent RNA 6000 Nano Kits (Agilent Technologies, Santa Clara, CA) and algorithmic interpretation on the Agilent 2100 Bioanalyzer© (Agilent Technologies). RNA samples were stained with an intercalating dye, separated by microcapillary electrophoresis on gel-filled microfluidic chips, and detected by laser-induced fluorescence creating an electropherogram.32 (link) The Bioanalyzer compares ribosomal RNA fragments (18S and 28S) of the sample electropherograms to a software algorithm generating an RIN. The quality of ribosomal RNA, as reflected by an RIN value, is utilized as an alternate measurement of messenger RNA (mRNA) quality.32 (link) The pH was determined on a 10% (wt:vol) sample (200 mg) of prefrontal cortex homogenized with a VWR pellet mixer in 2 mL Milli-Q water.
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RNA Quantification and Quality Assessment
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Corresponding Organization :
Other organizations : University of Maryland, Baltimore, Office of Chief Medical Examiner, Edward Via College of Osteopathic Medicine
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Variable analysis
independent variables
- None explicitly mentioned
- Concentration of RNA measured with Nanodrop 2000 UV-VIS spectrophotometer and expressed as nanograms per microliter
- Integrity of RNA samples assessed by Agilent RNA 6000 Nano Kits and Agilent 2100 Bioanalyzer, generating an RNA Integrity Number (RIN)
- PH of 10% (wt:vol) sample of prefrontal cortex homogenized in Milli-Q water
- None explicitly mentioned
- None mentioned
- None mentioned
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