Whole Blood mRNA Isolation and RT

Blood samples were immediately processed after collection as previously described (36 (link)). Whole blood was mixed with RNA Stabilization Reagent for Blood/Bone Marrow (Roche, Germany) for stabilization and stored at −20°C, in compliance with the instructions provided by the manufacturer. Next, samples were thawed at room temperature and mRNA was isolated using the mRNA Isolation Kit for Blood/Bone Marrow (Roche), according to the protocol provided by the manufacturer. Reverse Transcription (RT) was performed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

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Rodríguez-Carrio J., López P., Alperi-López M., Caminal-Montero L., Ballina-García F.J, & Suárez A. (2019). IRF4 and IRGs Delineate Clinically Relevant Gene Expression Signatures in Systemic Lupus Erythematosus and Rheumatoid Arthritis. Frontiers in Immunology, 9, 3085.

Publication 2018

RNA Stabilization Reagent for Blood/Bone Marrow mRNA Isolation Kit for Blood/Bone Marrow High-Capacity cDNA Reverse Transcription Kit

Blood Bone marrow Cdna Isolation Mrna Reverse transcription

Corresponding Organization : Universidad de Oviedo

Other organizations : Central University Hospital of Asturias

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Not explicitly mentioned

control variables

RNA Stabilization Reagent for Blood/Bone Marrow (Roche, Germany) for stabilization
Stored at −20°C, in compliance with the instructions provided by the manufacturer
MRNA Isolation Kit for Blood/Bone Marrow (Roche), according to the protocol provided by the manufacturer
High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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