qRT-PCR validation was performed for nine genes: three transcription factors involved in trophoblast differentiation, five differentially expressed target genes, and one housekeeping gene. The target genes were selected based on their placenta-specific or placenta-enriched expression, as revealed by earlier work [19 (link)]. Total RNA was reverse transcribed, and TaqMan assays (Table S10) were used for gene expression profiling on the Biomark high-throughput qRT-PCR system (Fluidigm, San Francisco, CA, USA).
Szilagyi A., Gelencser Z., Romero R., Xu Y., Kiraly P., Demeter A., Palhalmi J., Gyorffy B.A., Juhasz K., Hupuczi P., Kekesi K.A., Meinhardt G., Papp Z., Draghici S., Erez O., Tarca A.L., Knöfler M, & Than N.G. (2020). Placenta-Specific Genes, Their Regulation During Villous Trophoblast Differentiation and Dysregulation in Preterm Preeclampsia. International Journal of Molecular Sciences, 21(2), 628.
Corresponding Organization : Semmelweis University
Other organizations :
Michigan State University, Florida International University, University of Michigan–Ann Arbor, Detroit Medical Center, Eötvös Loránd University, Medical University of Vienna, Ben-Gurion University of the Negev
Expression levels of nine genes: three transcription factors involved in trophoblast differentiation, five differentially expressed target genes, and one housekeeping gene
control variables
None explicitly mentioned
controls
Positive control: Not specified
Negative control: Not specified
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