Isolation and Characterization of Outer Membrane Vesicles
Corresponding Organization :
Other organizations : Universidad Andrés Bello, Hospital Clínico de la Universidad de Chile
Protocol cited in 5 other protocols
Variable analysis
- None explicitly mentioned
- OMV yield, quantified by protein content (BCA assay) and lipid content (FM4-64 molecular probe), normalized by CFU/ml
- OMV size, measured as diameter from TEM images
- Bacteria growth in LB at 37°C with shaking (to OD600 = 1.1)
- Centrifugation at 5400 × g for 10 min at 4°C
- Filtration through 0.45 μm filter
- Ultrafiltration using 100 kDa Ultracel® ultrafiltration disks
- Ultracentrifugation at 150,000 × g for 3 h at 4°C
- None mentioned
- None mentioned
Annotations
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