Preparation of ePFe Extract for Analysis

The samples were prepared as described earlier by Wojdyło et al. [65 (link)]. A weighed sample of ePFe for in vitro analyses was dissolved in 80% MeOH, subjected to ultrasound treatment for 20 min (Sonic 6D; Polsonic, Warsaw, Poland), and centrifuged (MPW-55; Warsaw, Poland) at 19,000× g at 4 °C for 10 min to obtain a clear extract for use in the analyses.

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Siniawska M, & Wojdyło A. (2023). Polyphenol Profiling by LC QTOF/ESI-MS and Biological Activity of Purple Passion Fruit Epicarp Extract. Molecules, 28(18), 6711.

Publication 2023

Sonic 6D MPW-55

Ultrasound

Corresponding Organization : Wroclaw University of Environmental and Life Sciences

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Variable analysis

independent variables

EPFe sample preparation method (dissolved in 80% MeOH, subjected to ultrasound treatment for 20 min, and centrifuged at 19,000× g at 4 °C for 10 min)

dependent variables

Analytes/components measured in the clear extract obtained from the ePFe sample preparation

control variables

Centrifugation conditions (19,000× g at 4 °C for 10 min)
Ultrasound treatment duration (20 min)

controls

No positive or negative controls were explicitly mentioned in the protocol.

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