Three or four micron sections of human omentum were subject to immunohistochemistry using the aforementioned primary antibodies, as previously described23 (link). To quantify RNase 3 reactive cells in omentum, slides were scanned at 20x magnification (NanoZoomer, Hamamatsu, Japan) and analyzed with Aperio ImageScope software (Leica, Wetzlar, Germany). Large vessels were excluded. The Positive Pixel Count Algorithm was used to determine the fraction of RNase3+ cells among total cell nuclei, expressed as a percentage.
Pottanat N.D., Brook A.C., Bartosova M., Cortado H., Gupta S., Li B., Jackson A.R., Vonau M., Cohen S., Ferrara M., Ching C.B., Spencer J.D., Brauner A., Fraser D.J., Schmitt C.P., Eberl M., Ayoob R, & Becknell B. (2019). Analysis of the Ribonuclease A Superfamily of Antimicrobial Peptides in Patients Undergoing Chronic Peritoneal Dialysis. Scientific Reports, 9, 7753.
Corresponding Organization : University of Charleston
Other organizations :
Riley Hospital for Children, Indiana University – Purdue University Indianapolis, Cardiff University, The Ohio State University, Karolinska University Hospital, Karolinska Institutet
Fraction of RNase3+ cells among total cell nuclei (expressed as a percentage)
control variables
Thickness of omentum sections (kept constant at 3 or 4 microns)
Exclusion of large vessels
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
Annotations
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