IFN-β and IFN-γ Measurement in M. avium Infection

IFN-β production in mouse serum or BMMs culture supernatants at 24 or 72 hr post M.avium infection at 37°C and 5% CO₂ was measured. ELISA was performed according to the manufacturer’s instruction (ebioscience). Capture antibody (Purified anti-mouse IFN-β Antibody, Cat. 519202, Biolegend), detection antibody (Biotin anti-mouse IFN-β Antibody, Cat. 508105, Biolegend), IFN-β standard (Cat. 581309, Biolegend), Avidin-HRP (Cat.18-4100-94, ebioscience), and TMB (Cat.00-4201-56, ebioscience). IFN-γ in culture supernatant and lung homogenate was measured using IFN gamma ELISA kit (Cat. 88-7314-22; eBioscience). For in vivo IFN-γ measurement, mouse lung homogenate was prepared as described previously [35 (link)].