The human urinary bladder urothelial carcinoma cell line (J82) was procured from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cell culture conditions were Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum in a 5% CO2 atmosphere of a standard incubator.
WFA and apoptosis inhibitor Z-VAD-FMK (ZVAD) [20 (link)] were obtained from Selleckchem.com (Houston, TX, USA) and dissolved in dimethyl sulfoxide (DMSO). The antioxidant NAC (Sigma-Aldrich; St. Louis, MO, USA) was used to test the function of oxidative stress in WFA-induced changes. Cell survival was measured by the tetrazolium-based MTS kit (Promega Corporation, Madison, WI, USA) [21 (link)].
WFA and apoptosis inhibitor Z-VAD-FMK (ZVAD) [20 (link)] were obtained from Selleckchem.com (Houston, TX, USA) and dissolved in dimethyl sulfoxide (DMSO). The antioxidant NAC (Sigma-Aldrich; St. Louis, MO, USA) was used to test the function of oxidative stress in WFA-induced changes. Cell survival was measured by the tetrazolium-based MTS kit (Promega Corporation, Madison, WI, USA) [21 (link)].
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