Synthesis and Characterization of Silica-Coated SPIONs

Silica-coated SPIONs were synthesized by coprecipitation from aqueous solutions of iron salts, as described in detail elsewhere [81 (link),82 (link),83 (link)]. Then, the surface of the NPs was functionalized by addition of citric acid and coated with a ~4 nm thick silica shell as described in our published studies [84 (link),85 (link),86 (link),87 (link)]. The silica-coated SPIONs were characterized by TEM, DLS, and zeta potential measurements. Samples for TEM analysis were prepared by drying the aqueous suspension of silica-coated SPIONs at room temperature on a transparent carbon film on a copper grid, and images were acquired using a JEOL 2100 microscope (JEOL Ltd., Tokyo, Japan). Suspension of silica-coated SPIONs in serum-free medium (c = 1 mg/mL; pH = 7.91) was monitored by electrokinetic measurements of zeta potential (Anton Paar GmbH, Litesizer 500, Graz, Austria) and hydrodynamic diameter distribution was determined by DLS (Anton Paar GmbH, Litesizer 500, Graz, Austria).

Free full text: Click here

Repar N., Jovičić E.J., Kump A., Birarda G., Vaccari L., Erman A., Kralj S., Nemec S., Petan T, & Drobne D. (2022). Oleic Acid Protects Endothelial Cells from Silica-Coated Superparamagnetic Iron Oxide Nanoparticles (SPIONs)-Induced Oxidative Stress and Cell Death. International Journal of Molecular Sciences, 23(13), 6972.

Publication 2022

JEOL 2100 microscope Litesizer 500

Carbon Citric acid Copper Hydrodynamic Iron Microscope Salts Serum Silica Spions

Corresponding Organization : University of Ljubljana

Other organizations : Jožef Stefan International Postgraduate School, Jožef Stefan Institute, Elettra-Sincrotrone Trieste S.C.p.A.

Top 5 similar protocols

Variable analysis

independent variables

Synthesis method: Coprecipitation from aqueous solutions of iron salts
Surface functionalization: Addition of citric acid and coating with ~4 nm thick silica shell

dependent variables

Characterization of silica-coated SPIONs: TEM, DLS, and zeta potential measurements

control variables

Preparation of samples for TEM analysis: Drying the aqueous suspension of silica-coated SPIONs at room temperature on a transparent carbon film on a copper grid
Suspension of silica-coated SPIONs in serum-free medium (c = 1 mg/mL; pH = 7.91)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!