Western blotting was performed as described previously [15 (link)]. The antibodies against EZH2, PDCD4 and GAPDH were purchased from Cell Signaling Technology.
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Protocol detail
Western Blotting of EZH2, PDCD4, and GAPDH
Corresponding Organization : First Affiliated Hospital of Zhengzhou University
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Variable analysis
independent variables
- None explicitly mentioned
- Expression levels of EZH2, PDCD4, and GAPDH proteins
- Western blotting protocol as described previously [15]
- Antibodies against EZH2, PDCD4, and GAPDH from Cell Signaling Technology
- Positive control: None mentioned
- Negative control: None mentioned
Annotations
This protocol is too short. Unable to provide accurate annotations
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