Western Blotting for APC, Cyclin D1, and c-MYC

Western blotting was performed according to a previously reported method [17 (link)]. The membranes were probed with polyclonal mouse antibodies: anti-APC (ab15270; 1:1000; Abcam, Cambridge, UK), anti-cyclin D1 (1:1000; Cell Signaling Technology, Danvers, MA, USA), and anti-c-MYC (1:1000; Cell Signaling Technology). The membranes were stripped and re-probed with anti-α-tubulin mouse monoclonal antibody (1:1000; Cell Signaling Technology) as the loading control.

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Shu Z., Chen L, & Ding D. (2016). miR-582-5P induces colorectal cancer cell proliferation by targeting adenomatous polyposis coli. World Journal of Surgical Oncology, 14(1), 239.

Publication 2016

anti-APC (ab15270 anti-cyclin D1 anti-c-MYC anti-α-tubulin mouse monoclonal antibody

Antibodies Cyclin d1 Membranes Monoclonal antibody Mouse α tubulin

Corresponding Organization :

Other organizations : Jilin University, Union Hospital, Union Hospital

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Variable analysis

independent variables

Antibodies used for probing the membranes: anti-APC, anti-cyclin D1, and anti-c-MYC

dependent variables

Protein expression levels of APC, cyclin D1, and c-MYC

control variables

Loading control: anti-α-tubulin mouse monoclonal antibody

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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